The electrophoresis chip can realize on-line operation such as sample injection, sample accumulation and concentration, column front and back column derivation and so on. The chips are equipped with catheter connectors and other accessories, users only need syringes or pipettes to use. In order to standardize the conventional use of chips, all chip sizes use the size of glass slides or microholes, that is, the standard chip size is 25*76mm, and the distance between microchannels is 4.5mm or 9mm.