The fixture has a total of 4-layer structure, from the bottom up, followed by aluminum, glass, customer chips, PMMA board. The fixture uses aluminum plate and PMMA cover to exert pressure. The PDMS chip and glass / PMMA chip fit together. Fixture using reversible sealing means to avoid the chip sealing process, saving time and effort, easy chip cleaning. Fixture used with PEEK connector, injection simple, quick cleaning. Fixture with observation window, can be used with the microscope.