{"id":5353,"date":"2023-06-16T11:01:49","date_gmt":"2023-06-16T03:01:49","guid":{"rendered":"http:\/\/43.135.177.8\/?p=5353"},"modified":"2023-06-16T11:01:49","modified_gmt":"2023-06-16T03:01:49","slug":"a-novel-microfluidic-chip-for-rapid-screening-of-foodborne-pathogenic-bacteria","status":"publish","type":"post","link":"https:\/\/whmicro.com\/?p=5353","title":{"rendered":"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria"},"content":{"rendered":"<p>[vc_row rt_row_background_width=&#8221;default&#8221; rt_row_style=&#8221;default-style&#8221; rt_row_borders=&#8221;&#8221; rt_row_paddings=&#8221;true&#8221; rt_bg_effect=&#8221;classic&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;cover&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_bg_video_format=&#8221;self-hosted&#8221;][vc_column width=&#8221;4\/5&#8243; rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221;][vc_column_text]Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled &#8220;A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.&#8221;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"1685063201946454.png\" src=\"https:\/\/www.whchip.com\/upload\/202305\/1685063201946454.png\" alt=\"\u56fe\u72471.png\" width=\"719\" height=\"343\" data-width=\"719\" data-height=\"343\" \/><\/p>\n<p><span class=\"text-dst\">This study skillfully combines the interdisciplinary knowledge of engineering technology, biotechnology and nanomaterials, and uses the technologies of nuclear pore membrane separation and simulated enzyme catalysis to develop a new finger-pressed microfluidic chip without external power, which not only saves the cost of bacterial detection, but also simplifies the complex bacterial detection process.\u00a0<\/span><span class=\"text-dst\">This low-cost, easy-to-operate, highly sensitive and fast biosensor has the potential for field detection and can provide novel ideas for rapid screening of foodborne pathogenic bacteria.<\/span><\/p>\n<p><span class=\"text-dst\">In this study, on the microfluidic chip, through finger pressing operation, combined with nuclear pore membrane filtration, the sensitive detection of foodborne pathogens was realized in a short time.<\/span><\/p>\n<p><span class=\"text-dst\">First of all, the finger pressure mixer was used to mix the bacterial samples and immune gold and platinum particles (mimic enzyme), and the specific labeling of the target bacteria was realized.<\/span><\/p>\n<p><span class=\"text-dst\">Then, the bacterial-mimetic enzyme complex was filtered by nuclear pore membrane to separate the bacterial-mimetic enzyme complex from the excess mimetic enzyme.<\/span><\/p>\n<p><span class=\"text-dst\">Then, the intercepted bacterial-mimic enzyme complex was used to catalyze the mixed solution of hydrogen peroxide and tetramethylbenzidine, resulting in obvious color changes on the surface of the nuclear pore membrane.<\/span><\/p>\n<p><span class=\"text-dst\">Finally, the smartphone is used to take pictures and image processing, so as to calculate and analyze the number of target bacteria.\u00a0<\/span><span class=\"text-dst\">Through a simple finger-pressing microfluidic chip, the biosensor can complete the steps of sample mixing, bacterial separation, background cleaning, catalytic coloration and so on.<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" title=\"1685063224796511.png\" src=\"https:\/\/www.whchip.com\/upload\/202305\/1685063224796511.png\" alt=\"\u56fe\u72472.png\" width=\"741\" height=\"491\" data-width=\"741\" data-height=\"491\" \/><\/p>\n<p style=\"text-align: center;\"><strong>Schematic diagram of sensitive detection of foodborne pathogenic bacteria<\/strong><\/p>\n<p>\u00a0[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/5&#8243; rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221;][vc_widget_sidebar sidebar_id=&#8221;sidebar-for-portfolio&#8221;][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[vc_row rt_row_background_width=&#8221;default&#8221; rt_row_style=&#8221;default-style&#8221; rt_row_borders=&#8221;&#8221; rt_row_paddings=&#8221;true&#8221; rt_bg_effect=&#8221;classic&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;cover&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_bg_video_format=&#8221;self-hosted&#8221;][vc_column width=&#8221;4\/5&#8243; rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221;][vc_column_text]Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":5354,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v18.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao<\/title>\n<meta name=\"description\" content=\"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled &quot;A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.&quot;\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/whmicro.com\/?p=5353\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao\" \/>\n<meta property=\"og:description\" content=\"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled &quot;A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.&quot;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/whmicro.com\/?p=5353\" \/>\n<meta property=\"og:site_name\" content=\"WenHao\" \/>\n<meta property=\"article:published_time\" content=\"2023-06-16T03:01:49+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"741\" \/>\n\t<meta property=\"og:image:height\" content=\"491\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Happy\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/whmicro.com\/#website\",\"url\":\"https:\/\/whmicro.com\/\",\"name\":\"WenHao\",\"description\":\"Microfluidic Chip &amp; Device Manufacturer\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/whmicro.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/whmicro.com\/?p=5353#primaryimage\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png\",\"contentUrl\":\"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png\",\"width\":741,\"height\":491},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/whmicro.com\/?p=5353#webpage\",\"url\":\"https:\/\/whmicro.com\/?p=5353\",\"name\":\"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao\",\"isPartOf\":{\"@id\":\"https:\/\/whmicro.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/whmicro.com\/?p=5353#primaryimage\"},\"datePublished\":\"2023-06-16T03:01:49+00:00\",\"dateModified\":\"2023-06-16T03:01:49+00:00\",\"author\":{\"@id\":\"https:\/\/whmicro.com\/#\/schema\/person\/0a1c1029820a65bb7260d8eb6629140a\"},\"description\":\"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled \\\"A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.\\\"\",\"breadcrumb\":{\"@id\":\"https:\/\/whmicro.com\/?p=5353#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/whmicro.com\/?p=5353\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/whmicro.com\/?p=5353#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"\u9996\u9875\",\"item\":\"https:\/\/whmicro.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria\"}]},{\"@type\":\"Person\",\"@id\":\"https:\/\/whmicro.com\/#\/schema\/person\/0a1c1029820a65bb7260d8eb6629140a\",\"name\":\"Happy\",\"image\":{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/whmicro.com\/#personlogo\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/ea4fb3ed9439d0adf14051b1ee30e2c4?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/ea4fb3ed9439d0adf14051b1ee30e2c4?s=96&d=mm&r=g\",\"caption\":\"Happy\"},\"sameAs\":[\"http:\/\/43.135.177.8\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao","description":"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled \"A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.\"","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/whmicro.com\/?p=5353","og_locale":"en_US","og_type":"article","og_title":"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao","og_description":"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled \"A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.\"","og_url":"https:\/\/whmicro.com\/?p=5353","og_site_name":"WenHao","article_published_time":"2023-06-16T03:01:49+00:00","og_image":[{"width":741,"height":491,"url":"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Written by":"Happy","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebSite","@id":"https:\/\/whmicro.com\/#website","url":"https:\/\/whmicro.com\/","name":"WenHao","description":"Microfluidic Chip &amp; Device Manufacturer","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/whmicro.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"ImageObject","@id":"https:\/\/whmicro.com\/?p=5353#primaryimage","inLanguage":"en-US","url":"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png","contentUrl":"https:\/\/whmicro.com\/wp-content\/uploads\/2023\/06\/2-1.png","width":741,"height":491},{"@type":"WebPage","@id":"https:\/\/whmicro.com\/?p=5353#webpage","url":"https:\/\/whmicro.com\/?p=5353","name":"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria - WenHao","isPartOf":{"@id":"https:\/\/whmicro.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/whmicro.com\/?p=5353#primaryimage"},"datePublished":"2023-06-16T03:01:49+00:00","dateModified":"2023-06-16T03:01:49+00:00","author":{"@id":"https:\/\/whmicro.com\/#\/schema\/person\/0a1c1029820a65bb7260d8eb6629140a"},"description":"Recently, the team of Professor Lin Jianhan of the School of Information and Electrical Engineering of China Agricultural University published the latest research results in the famous journal biosensor and Bioelectronics (Biosensors and Bioelectronics) in the sensor field, entitled \"A biosensor for detection of Salmonella typhimurium based on finger pressing mixing and nuclear pore membrane separation.\"","breadcrumb":{"@id":"https:\/\/whmicro.com\/?p=5353#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/whmicro.com\/?p=5353"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/whmicro.com\/?p=5353#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"\u9996\u9875","item":"https:\/\/whmicro.com\/"},{"@type":"ListItem","position":2,"name":"A novel microfluidic chip for rapid screening of foodborne pathogenic bacteria"}]},{"@type":"Person","@id":"https:\/\/whmicro.com\/#\/schema\/person\/0a1c1029820a65bb7260d8eb6629140a","name":"Happy","image":{"@type":"ImageObject","@id":"https:\/\/whmicro.com\/#personlogo","inLanguage":"en-US","url":"https:\/\/secure.gravatar.com\/avatar\/ea4fb3ed9439d0adf14051b1ee30e2c4?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/ea4fb3ed9439d0adf14051b1ee30e2c4?s=96&d=mm&r=g","caption":"Happy"},"sameAs":["http:\/\/43.135.177.8\/"]}]}},"_links":{"self":[{"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/posts\/5353"}],"collection":[{"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/whmicro.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5353"}],"version-history":[{"count":1,"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/posts\/5353\/revisions"}],"predecessor-version":[{"id":5355,"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/posts\/5353\/revisions\/5355"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/whmicro.com\/index.php?rest_route=\/wp\/v2\/media\/5354"}],"wp:attachment":[{"href":"https:\/\/whmicro.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5353"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/whmicro.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5353"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/whmicro.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5353"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}