{"id":5350,"date":"2023-06-16T10:36:53","date_gmt":"2023-06-16T02:36:53","guid":{"rendered":"http:\/\/43.135.177.8\/?p=5350"},"modified":"2023-06-16T10:36:53","modified_gmt":"2023-06-16T02:36:53","slug":"how-to-obtain-single-cell-individuals-by-droplet-microfluidic","status":"publish","type":"post","link":"https:\/\/whmicro.com\/?p=5350","title":{"rendered":"How to obtain single-cell individuals by droplet microfluidic"},"content":{"rendered":"

[vc_row rt_row_background_width=”default” rt_row_style=”default-style” rt_row_borders=”” rt_row_paddings=”true” rt_bg_effect=”classic” rt_bg_image_repeat=”repeat” rt_bg_size=”cover” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_bg_video_format=”self-hosted”][vc_column width=”4\/5″ rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll”][vc_column_text]A variety of mutated strains of COVID-19 are raging all over the world. in the face of the constantly mutating virus, scientists are still working day and night on the pathogenic mechanism of the virus, potential therapeutic targets and screening effective antibodies.<\/p>\n

Single cell study<\/strong><\/p>\n

Cell research is one of the hotspots in the field of biotechnology.\u00a0<\/span>The object of traditional cell biology analysis is usually hundreds of thousands to millions of cells.\u00a0<\/span>With the development of modern biology, scientists tend to study the diversity between cells.<\/span><\/p>\n

This can not only fully understand some special cellular functions in the cell population, but also identify a small amount of diversity in a large number of cell populations. it provides a new technical approach for early diagnosis and treatment of major diseases, drug screening and cell-cell interaction.<\/span><\/p>\n

Microfluidic single cell analysis technique.<\/span><\/strong><\/p>\n

In recent years, microfluidic is often used in laboratory for single cell research, but in microfluidic single cell analysis, single cell capture is also needed, that is, a single cell is separated from the micron channel and fixed in a specific position. in order to facilitate the follow-up operation and analysis.<\/span><\/p>\n

Droplet microfluidic technology provides further support for the detection, sorting and analysis of single cells, that is, through the mechanical action of two-phase or multiphase immiscible liquids in micron-level pipes, a series of nanoliter-to-skin upgrade droplets are generated.<\/span><\/p>\n

In the single cell coating experiment using droplet microfluidic control, the buffer containing cells interacted with the oil phase liquid to form uniform cell buffer droplets in the oil phase, and each cell was wrapped in the droplet.\u00a0<\/span>This technique can not only produce a large number of single-cell droplets in a short time, but also each droplet can be used as an independent single-cell microreactor, which can effectively avoid cross-contamination and meet the final research needs of a variety of single cells.<\/span><\/p>\n

In the experiment, the common droplet generation system mainly includes three parts: microinjection pump, microfluidic chip and high-throughput screening system.\u00a0<\/span>The generation of microdroplets with uniform size and stable velocity is the key to the normal operation of the system.\u00a0<\/span>As the power source of the whole system, the micro-injection pump provides stable micro-fluid flow and stable and fine power for the production of micro-droplets.<\/span><\/p>\n

\"\u56fe\u72471.png\"<\/p>\n

Microinjection pump, microfluidic chip and high throughput screening system<\/strong><\/p>\n

 <\/p>\n

Application case.<\/span><\/p>\n

The single cell encapsulation experiment was carried out by droplet microfluidic technology in a medical laboratory of a university in China.\u00a0<\/span>Many kinds of liquids need to be transported in the laboratory, so Lange multiple injection pumps are used for accurate and stable liquid transmission.<\/span><\/p>\n

\"1629688407881638.gif\"<\/p>\n

The formation process of microdroplets under microscope<\/p>\n

\"\u56fe\u72472.png\"\"\u56fe\u72473.png\"<\/p>\n

 <\/p>\n

The cells are wrapped in droplets observed under a microscope.<\/span><\/p>\n

Wenhao WH-PSP-08 high-precision laboratory fuel injection pump, accurate distribution, strong anti-interference ability.\u00a0<\/span>Driven by the jet pump, the liquid flow artery has small movement and good stationarity, which can provide stable microfluidic flow in the microfluidic system.<\/span><\/p>\n

\"\u56fe\u72474.png\"[\/vc_column_text][\/vc_column][vc_column width=”1\/5″ rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll”][vc_widget_sidebar sidebar_id=”sidebar-for-portfolio”][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"

[vc_row rt_row_background_width=”default” rt_row_style=”default-style” rt_row_borders=”” rt_row_paddings=”true” rt_bg_effect=”classic” rt_bg_image_repeat=”repeat” rt_bg_size=”cover” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_bg_video_format=”self-hosted”][vc_column width=”4\/5″ rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll”][vc_column_text]A variety of mutated strains of COVID-19 are raging all over the world. in the face of the constantly mutating virus, scientists are still working day and night […]<\/p>\n","protected":false},"author":1,"featured_media":5351,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"yoast_head":"\nHow to obtain single-cell individuals by droplet microfluidic - 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